Plant viruses cannot penetrate the intact plant cuticle and cellulose cell wall that acts as barrier to infection. The virus overcomes the problem by either avoiding the need to penetrate (example seed transmission) or by using the wound in plants as infection site, or transmission by insects, nematodes or fungi as a vector.  
 
Mechanical transmission involves the introduction of infective virus or viral RNA into the wounds of plants. Viruses such as Tobacco mosaic virus (TMV), Potato virus X are highly stable, and reach high concentration in plants.  As you all know TMV can readily contaminate hands, clothings, and implements and can be spread by worker. TMV can even spread mechanically by tobacco smokers as the virus is present in cured tobacco leaves. 
Mechanical transmission is of great importance.  In field and greenhouse, great amount of caution has to be implemented to not transmit the infection. Field sanitation, tool sanitation is very important to avoid the spread of virus. 
However, in experimental world mechanical transmission is a very useful tool to study viruses. Mechanical inoculation of virus to a heathy host plant is done for assays, to produce local lesions, in the propagation to of viruses for purification, in host range study, diagnosis, and to understand the interaction between virus and susceptible cells. 
 
 
Seed transmission:  About 1/7 th of the known plant viruses are transmitted through seeds. Different viruses have different host ranges (the plants that they can infect). Tobacco mosaic virus, Cucumber mosaic virus are some viruses with a very wide host range and they may not be seed transmissible in all plants they infect. Seed transmission plays a huge role in virus epidemiology. Not only they can be a primary source of infection, leading to an epidemic in the field upon conducible environment, seed transmission is an effective way for long distance travel of the virus, thus introducing the virus to new places. You have heard of USDA regulations/restrictions on different crops, from certain foreign countries to avoid introduction of infected seeds/plant materials. 
 
Seed transmission can occur simply by contamination of seeds, as in tomato seeds by Tobacco mosaic virus. This can be readily inactivated by seed treatments. 
The second type of seed transmission occurs when the virus is present in the embryo tissue that can happen prior to fertilization or takes place at pollination. Pea seed-borne mosaic virus is a well studied plant virus in this category. 
 
 
Pollen Transmisison:  Some viruses are transmitted from plant to plant via pollen. As in seed transmission, pollen transmission has two mechanisms, gametic infection of embryo and direct infection of mother plant. 
 
Vegetative propagation: An important horticultural practice, and unfortunately a very effective method for perpetuating and spreading viruses. In clonally propagated plants, an infected mother plant which could be asymptomatic could be used to make hundreds and thousands of daughter plants, which will all have the virus. Any vegetative parts  such as bulbs, corms, runners, and cutting will be infected. 
 
Grafting: Essentially a form of vegetative propagation, once the organic union has been established and plants (Scion and Stock) function as a single plant. In experimental front, grafting is used as a virus transmission methods, when all other methods fail.

Steam sterilization of soils is commonly used in plant nurseries and greenhouses for effective control of soilborne pathogens and weed seeds. The technique, however, is highly energy intensive as the entire soil profile is heated. This is too costly and slow to be practical for field scale vegetable production.  To reduce energy consumption and cost, use of band-steaming, where steam is applied only in the area where it is needed – in the plant root zone, is proposed. In this method, narrow strips of soil centered on the seed line are treated with steam rather than the whole bed.

Over the course of the last year, we developed a prototype band-steam and co-product applicator that is designed to raise soil temperatures in a band 2” deep by 4” wide to levels sufficient to control soilborne pathogens (140 °F for > 20 minutes) and weed seed (150 °F for > 20 minutes). The device is principally comprised of a 35 BHP steam generator and a co-product applicator mounted on top of a bed shaper (Fig.1). The apparatus applies steam via shank injection and from cone shaped ports on top of the bed shaper. An exothermic compound can be co-applied via shank injection and/or a banding spray nozzle. The rationale behind co-applying an exothermic compound with steam is that exothermic compounds react and release heat when combined with water, thereby reducing energy requirements and increasing travel speed.

Preliminary testing of the device this spring in Yuma, AZ were very promising. Trial results showed that application of steam alone effectively raised soil temperature in the center of the seed line to levels required for effective pest control (140 °F for more than 20 minutes). Use of the exothermic compound increased soil temperature by about 10 °F. A video of the device in action can be found at the link provided below.

We are currently evaluating the device in field trials with lettuce in Salinas, CA. Target pests in these experiments conducted in collaboration with Steve Fennimore, UC Davis, are soil pathogens which cause Sclerotinia lettuce drop and in-row weeds. Future articles will report the findings of this research.

This fall, we will be replicating these tests in Yuma, AZ and also investigating the effectiveness of band-steam for controlling Fusarium oxysporum f. sp. lactucae which causes Fusarium wilt of lettuce. Heat has been shown to effectively kill Fusarium oxysporum spores and control Fusarium wilt disease. As an example, soil solarization, where clear plastic is placed over crop beds during the summer, raises soil temperatures to 150-155˚F at the soil surface, effectively killing the pathogen and reducing disease incidence by 45-98% (Matheron and Porchas, 2010). 

These projects are sponsored by USDA-NIFA, the Arizona Specialty Crop Block Grant Program and the Arizona Iceberg Lettuce Research Council.  We greatly appreciate their support.

If you are interested in seeing the machine operate or would like more information, please feel free to contact me.

See the band-steam and co-product applicator in action!

References: Matheron, M. E., & Porchas, M. 2010. Evaluation of soil solarization and flooding as management tools for Fusarium wilt of lettuce. Plant Dis. 94:1323-1328.

Sprangletop has become increasingly widespread in Arizona mostly because of its growth habits and tolerance to many commonly used herbicides. It is in the Leptochloa genus which is derived from the Greek words leptos (thin) and chloa (grass). There are more than 150 species of sprangletop worldwide but only three in Arizona and two in Yuma County. The two that are the most common in the low desert are Mexican Sprangletop, which is Leptochloa uninervia and Red Sprangletop, Leptochloa filiformis. A third species, Bearded Sprangletop, Leptochloa fascicularis, is more common at higher elevations of 1500 feet or higher. It is not uncommon to find both Red and Mexican Sprangletop in the same field and it is not hard to distinguish them when they are side by side. Red Sprangletop has a light green leaf blade which is similar in width to watergrass and barnyardgrass. It has very fine hairs and very small and fine branches and spiklets. It also has a long membranous ligule. The name Red refers to the leaf sheath, which is characteristically red, rather than the seed head. Mexican Sprangletop has a thinner leaf blade which is darker green or grayish in color and similar in appearance to common bermudagrass. The seed head is distinctly coarser than that of Red Sprangletop. Side by side, leaf color and size of the seed make it easy to distinguish these two. Both of these grasses are classified as summer annuals, but they grow more like perennials in the low desert. Sprangletop does very well in the hottest part of the summer and typically germinates from seed during the hottest period between July and September. Once established, however, it often survives through the cold winter months. It grows into clumps that often appear to be dead during the winter. New shoots commonly grow from these established crowns the next season. When this occurs, preemergent herbicides such as Trifluralin or Prowl are ineffective. Some Sprangletop plants stay green and grow through the winter. Many of the postemergence, grass specific herbicides that control many grasses are ineffective on Sprangletop. This also has contributed to the spread of these weeds. Sethoxydim (Poast) and Fluazifop (Fusilade) do not control either Red or Mexican sprangletop. Only Clethodim (Select Max, Select, Arrow and others) is the only one of these grass herbicides that is effective and only at the highest labeled rates. Two applications are often necessary to achieve season long control.