May 5, 2021Summer Sanitation Is Important as Ever
To contact John Palumbo go to: jpalumbo@ag.Arizona.edu
In the past couple of weeks, the reports of INSV in fields has increased dramatically. INSV has been found in fields in Yuma/Gila Valley, Wellton, Tacna, Roll, and Imperial Valley.
PCAs have reported thrips pressure as low this year and most fields have infection less than 1% but some fields have been reported to have higher incidence. The virus has been detected in direct seeded field as well as transplants imported from Salinas, CA.
Impatiens necrotic spot virus, also known as INSV is a tospovirus closely related to Tomato spotted wilt virus. Infected plants usually have leaves with brown to dark brown necrotic areas. Sometimes the symptoms may be confused with “chemical burn”. As necrosis progresses the leaf browns or die out. Plants infected in early stage may become stunted and die, or become unmarketable.
What makes this virus of high economic importance?
The plants become unmarketable which is the ultimate economic loss. But there are factors that facilitate the virus outbreak.
The first one is efficient transmission by its vector (s). The virus is transmitted by western flower thrips, Frankliniella occidentalis.
If you remember our virus transmission series in past newsletters, thrips transmit viruses in persistent propagative manner. Insects have to feed on virus infected plants for hours/days to acquire the virus and the virus has to incubate for hours/days in the insect. After insect can transmit the virus throughout its lifespan. The virus can multiply in the vector system and often times the virus particles are also passed on to the insect offspring. Adult thrips can transmit these viruses only if acquired in the larval stage of development. Larval thrips will feed on a virus-infected plant, pupate, and emerge as a winged adult capable of transmitting the virus. The thrips then will carry the virus for life.
The next contributing factor is host range. INSV infects large number of ornamental and vegetable plants. We are talking 600 species of plants that are susceptible to INSV and thrips love flowers.
While it may not be practical to remove all your ornamentals in fear of INSV, it is definitely practical to monitor thrips population in your field. As the legend says “When in doubt, scout”.
And if you need diagnosis, drop the samples in the clinic! But then if you have immunostrips, you don’t have to make the drive to the Ag Center!
DIY testing: Impatiens necrotic spot virus (INSV)
As visual diagnosis of the virus is confusing and could even be misleading at times, it is very important to confirm a symptomatology via clinical diagnosis.
The good news is there are tools available for quick and easy diagnosis of INSV. You can order the immunostrips from Agdia (https://orders.agdia.com/agdia-immunostrip-for-insv-isk-20501)
The immunostrips cost anywhere from $5-20 depending on how much you buy. They perform better when they stay refrigerated until just before use.
Immunostrips are quick and easy tool to use. The kit comes with a buffer bag and immunostrip.
One band means that the positive control worked which means the system worked. Sometimes you see no bands at all. This means the system did not work and you have to repeat the test.
If you are seeing symptoms in your field please let Bindu Poudel-Ward know via email (firstname.lastname@example.org or text (928-920-1110). Please keep a note of weed species you are consistently seeing in your fields and keep the thrips population under check.
Weed escapes are easy to spot in vegetable fields at harvest time. Some growers have these weeds pulled, bagged and removed by hand from the field because they are unsightly and to reduce seedbank loads. This can be a costly operation. An alternative solution might be to use high voltage electricity to kill these weeds. The idea of using electricity to “zap” weeds is not new. Machines for agriculture applications were developed decades ago and commercially available in the late 1970’s. Although the devices worked, they were not widely adopted due in part to the availability of low cost and efficacious herbicides.
Because of environmental concerns, herbicide resistant weed issues and increased organic production, non-chemical, high voltage weed control technology is seeing a resurgence. There are now five companies, three established within the last four years, offering or developing machines for commercial agriculture. Although configurations differ, all machines operate using the same principles. To explain, consider the example of the machine shown in Fig. 1. The unit comprises high voltage electrodes (8-15 kV) positioned above the crop canopy, an electric generator and a soil engaging coulter connected to ground. During operation, when an electrode touches a weed protruding above the canopy, current flows through the plant back to the generator via the ground contacting coulter. Current flow combined with electrical resistance in the plant causes rapid heating and plant fluids to vaporize. This ruptures cell walls and kills the plant. Although there are few recent reports in the literature, prior research on dated machines showed that the technique can provide better than 98% weed control in moderate weed densities (15,000 weeds/acre) at travel speeds of 2 mph (Diprose & Benson, 1984).
Modern approaches that utilize high voltage electricity in combination with smart machines to spot treat weeds are being developed. The idea is to use camera imagery and artificial intelligence to locate weeds and high voltage electricity to kill them. One such machine being developed by the MASCOR Institute1 and the Zasso Group is an autonomous robot equipped with cameras, on-board computers and robotic arms (Fig. 2). As the machine moves through the field, high voltage electrodes mounted on the movable, computer controlled robotic arms zap weeds. Another unit is being developed by Stekettee and RootWave. It is tractor pulled and designed to travel at 3 mph. Stekettee’s machine vision system identifies the weeds and RootWave’s high voltage electric technology shocks the weed with a pulsed 5 kV charge. Power is supplied by a generator connected to the tractor’s PTO. Both systems are in late stages of development with field tests conducted in 2020.
These systems appear promising and if they prove to be effective and economical, may be something to look for in the future.
1Reference to a product or company is for specific information only and does not endorse or recommend that product or company to the exclusion of others that may be suitable.
The Yuma County Leaf Wetness Network remains in place for the 2018/19 vegetable season. Growers and PCAs may access information generated by the network by entering the following internet address: http://188.8.131.52:460
Upon entering the address above, you will be transferred to internet page that provides a series of tabs at the top of the page. Simply click on the tabs to access the information of interest.