Viruses vectored by insects 
 
We are on the final section of virus transmission. Virus transmission by insects is one of the most efficient and economically important transmission in agriculture. When you have insects in your crops, not only you are losing your crops because of feeding/chewing by insects, a lot of insects also act as a vector of plant viruses. 
 
Seven out of 29 orders of insect feeding on living green land plants are vectors of plant viruses. 
Insect transmit viruses in 4 distinct modes: 
 

1. Non persistent transmission: The insects can acquire the virus in a matter if seconds/minutes and they are immediately viruliferous. The virus in retained in the stylet of the insect and are transmitted to the next plant the insect feeds on. The virus is retained in the vector only for few minutes and is lost after insect molting. Most viruses transmitted by aphids are non persistent. So when you see few aphids in your melon field and see cucumber mosaic virus symptoms 1-2 weeks later in your field, don’t be surprised. Aphids are efficient vectors, and since viruses are systemic it takes anywhere from few days to 2-3 weeks for the plants to show symptoms. Thus it is very important to manage insects in the field even if you don’t think the ‘pressure’ is not as high. 

Viruses transmitted in non-persistent manner:
 Papaya ringspot virus 
Zucchini yellow mosaic virus 
Watermelon mosaic virus

1. Semi-persistent transmission: The insects can acquire the virus in minutes/hours and there is no latent (incubation) period in the insect. The virus can stay in the insects foregut for hours and is lost after insect molting. Some species of aphids and whiteflies fall in this category.  Example: Cucurbit yellow stunting disorder virus in melons transmitted by whiteflies. 

1. Persistent circulative: Insects have to feed on virus infected plants for hours/days to acquire the virus and the virus has to incubate for hours/days in the insect. After insect can transmit the virus for weeks. Virus can be present in the vectors hemolymph but there is no multiplication of virus in the insect body. Vectors in this transmission includes: Aphids, leafhopper, whiteflies, treehopper. 

Example: Beet curly top virus transmission by beet leafhopper 
 

1. Persistent propagative:  Insects have to feed on virus infected plants for hours/days to acquire the virus and the virus has to incubate for hours/days in the insect. After insect can transmit the virus throughout its lifespan. The virus can multiply in the vector system and often times the virus particles are also passed on to the insect offspring. Tomato spotted wilt virus is transmitted on persistent propagative manner by 9 different species on thrips. 

Save the Date : 2024 Plant Pathology Workshop 
When: August 29^th 8AM-12 PM ( breakfast and Lunch provided by Gowan Company and BASF) 
Where: Yuma Ag Center, 6425 W 8^th Street 
What will covered: Plant Pathology program Updates, past season field trial results (we
have some exciting results to share), Q&A to help better Plant pathology program,
Industry panel discussion for all your industry related questions! See you in few weeks! 

Automated thinning machines were first commercialized in 2012, almost a decade ago. Since then, the machines have improved tremendously, and automated thinning has become the standard practice in the Yuma area. As such, I was a bit surprised to see numerous fields this year with sub-optimal thinning performance. Many fields had numerous amounts of excess seedlings that should have been thinned and needed to be removed during the follow-up hand weeding/double removal operation (Fig. 1). There were also some fields where the machine was apparently not readily detecting seedlings, resulting in long sections of plants being killed and a poor final stand (Fig. 2).

Automated thinning machines can and do perform very well when set-up and calibrated correctly. However, field conditions such as lighting, levels of crop residue, weed pressure or soil type (color) change throughout the day and affect the way the machine chooses which plants to selectively thin.

As such, it may be beneficial to establish a protocol where automated thinning machine settings are checked at regular intervals throughout the day to ensure the unit is performing optimally. Doing so may help lower follow-up hand weeding/double removal costs and minimize the number of unnecessary skips in the field.

Fig. 1. Labor crew removing excess lettuce seedlings and weeds after machine thinning.


Fig. 2. Poor final stand establishment following machine thinning, possibly due to incorrect machine calibration.

Preemergence herbicides can be very effective in the low deserts where weeds germinate with every irrigation or rainfall. It is essential, however, that they be at the right place in the soil at the right time. The right place is in the soil around the weed seeds and the right time is when they are germinating. Only fumigants kill seeds. For most other herbicides, the seed must germinate and are then killed. When weed seeds germinate is dependent on a great number of interdependent variables.  These include weed species, soil type, moisture, soil temperature, condition of the seed, depth, organic matter and many other variables. Many seeds have biochemical mechanisms that allow them to germinate at different times. Some may germinate in a few hours and some may not germinate for several years. If they all germinated at the same time, they would be easier to control. The methods used to predict when weed seeds will germinate vary from very sophisticated numeric equations to Farmers Almanac type lore involving things like the thickness of gopher fur, the cuticle thickness of plants or the condition of bird feathers. Some methods will give you a precise day while others will give you just a general idea. In general, as the precision goes up, the accuracy goes down. Regardless of what technique you use, now is the best time to apply preemergence herbicides for the control of annual weeds. It is better to be a month early than a day late.We have conducted trials to determine how long it takes various weed species to germinate after receiving moisture. Results will vary depending on several factors and from field to field and year to year. Results are summarized below for individual species and by month. An average of all species is also included.
   Hours to Germination

NG = No germination