May 13, 2020
Virus Transmission: Part II
Fungal transmission of viruses:
Several viruses have been shown to be transmitted by soil-inhabiting fungi and protists. Two of the common fungal species that transmit viruses in vegetable cropping system are Olpidium spp. and Polymyxa spp. There is specificity between the zoospores of the fungi and virus particles for successful transmission to occur. Not all soilborne fungi can transmit viruses.
Olipdium brassicae is an obligate parasite, meaning the fungus always needs a host plant to survive (brassicae, cucumber, carrot, lettuce). The fungius that act as vector of s viruses survive the time in between crops by producing resting spores. A virus that we often see in the desert, Lettuce big vein virus, is transmitted fungus Olpidium brassicae . Lettuce big vein virus is acquired by the zoospore (motile spores) as well as resting spores of the fungus.
The other soilborne virus we see in the desert it Tomato bushy stunt virus is soilborne but has no known vectors. Thought it was predicted to be transmitted by a fungus Olpidium brassicae, new research findings have rules out the particular fungal vector. But there is a possibility that the virus has some soilborne vector.
Common viruses with fungal vector:
Cucumber necrosis virus: Olpidium bornovanus
Beet necrotic yellow vein virus: Polymyxa betae
Beet soilborne mosaic virus: Polymyxa betae
Beet soilborne virus: Polymyxa betae
Beet virus Q: Polymyxa betae
Nematode transmission of viruses
Two genera of viruses; Nepoviruses (Nematode transmitted polyhedral viruses) and Tobraviuses (Tobacco rattle virus) are transmitted by nematodes. Xiphinema (Dagger nematodes), Longidorus (needle nematodes), Trichodorus and Paratrichodorus (stubby root nematode) vector transmit different species of viruses.
Virus transmission by nematodes happens in 7 different but interrelated steps.
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1. Ingestion: The process where the nematode ingests the virus particle along with the plant material/sap.
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2. Acquisiton: The unique receptor in the nematode identifies the virus, so he virus particles stay intact within the nematode.
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3. Adsorption: The phenomena of the virus being intact instead of disintegrated in the nematode digestive system.
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4. Retention: Once adsorption, the virus particle can be retained in nematodes anywhere from months to even years.
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5. Release: The virus particles are released onto new plants when nematodes commences feeding on new plant.
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6. Transfer: The virus particles get transferred to new plant.
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7. Establishment: The virus particles establish themselves in the new host plant.
It is a sophisticated and thankfully a very specific relationship. Not all nematodes transmit virus, neither do nematodes vector all plant viruses. Nematodes and nematode transmitted viruses are of a bigger problem in perennial agriculture system such as citrus orchards and grapevines.
Viruses transmitted by nematodes:
tobacco rattle virus: beans, beets, peppers, potatoes, and spinach and 100 others tomato ringspot virus-tomato, cucumbers, peach rosette mosaic virus: peach, blueberries cherry
rasp leaf virus: cherry, apple
grapevine fanleaf virus: grapes and grape hybrids
To contact Bindu Poudel go to:
bpoudel@email.arizona.edu