Fall melon planting is right around the corner and local fields are being prepared for planting. Growers and PCAs are well aware of cucurbit yellow stunting disorder virus (CYSDV) and the impact it can have on fall melons. This whitefly transmitted crinivirus was first identified on desert melons in the fall 2006 where widespread infections on cantaloupes, honeydews and other melons occurred. CYSDV can cause significant losses in melon fruit yield and quality, and without question, desert melon crops have been seriously affected by this virus. Melon IPM has also been impacted by CYSDV where insecticide usage on fall melons has increased significantly. We have been studying the epidemiology of CYSDV for over 10 years trying to understand the complex relationships between the virus, vector and our local cropping system. Our ultimate goal is to develop practical approaches for reducing CYSDV impact on fall melon production. In addition, we continue to develop new information on chemical control of the whitefly vector (Bemisia whitefly adults).
Last fall, whitefly populations were lighter than normal, and CYSDV incidence on fall melons was the lowest we’ve recorded on melons since the virus as first reported 11 years ago. Thus far, whitefly numbers this spring and summer have been relatively light compared to previous years and the incidence of CYSDV was generally light on spring melons. How these low numbers translate into virus incidence on the fall melon crop is unknown? Regardless, experience suggests that growers should anticipate CYSDV to be present. Further, given the aggressive management programs that PCAs and growers are now using, it will be interesting to see how CYSDV impacts melon production this fall. We’ll be tracking both whiteflies and CYSDV incidence again in 2017. Our research to date suggests that fall melons produced near cotton or near areas where spring melons were recently produced are at the highest risk of infection. When possible, growers should attempt to isolate fall melon plantings as far away as possible from these sources of whiteflies and CYSDV. Growers forced to plant fall melons near these crops should be vigilant in minimizing adult whitefly infestation levels with insecticides during pre-bloom growth stages. To view a summary of the status of CYSDV in Yuma County and guidelines for management visit 2017 Guidelines for Whitefly and CYSDV Management on Fall Melons.
In response to the recent outbreaks of Diamondback moth (DBM) , Plutella xylostella in Yuma, we have established a pheromone trap network designed to monitor the activity and movement of adult populations of DBM. PCAs have had difficulty controlling DBM in cabbage, broccoli and cauliflower since October. Traps have been placed in Roll, Wellton, Dome Valley, Gila Valley and Yuma Valley in locations where cole crops are presently being grown or in areas where infestations were known to occur this fall.
Widely accepted definition of a living organism “A living organism has a cellular structure and is manifest by growth through metabolism, reproduction, and the power of adaptation to the environment through changes that originate internally”. Viruses are not cellular and do not metabolise, but they reproduce and adapt.
A virus is a set of one or more nucleic acid template molecules, normally incased in a protective coats of protein or lipoprotein and is able to organize its own replication but only within a suitable host cells. Record of plant viruses do not go as far as human viruses, but plant viruses have caused considerable loss in agriculture system.
One of the most common virus we see in agriculture system in todays world is Cucumber mosaic virus(CMV). CMV belongs to family Bromoviridae. The genome size of cucumber mosaic virus (see pic) is about 8000 to 9000 nucletotide bases (1 base=1 letter of AGTC). The genome size of Covid19 Coronivirus is about 30,000 bases and the genome size of human DNA is 6.4 billion bases.
CMV has a very wide host range and is transmitted by aphids in nonpersistent manner (stylet borne). This means that the aphids acquire the virus particle in their stylet within seconds of feeding in infected plants, hop on to next plant and start feeding on next plant. The virus is transmitted to the next plant immediately.
Next is incubation period. Viruses cause systemic infection. It can take anywhere from few days to few weeks from initial entry of the virus to symptom exhibition in your plants. The severity of symptoms varies depending on many factors. The age of plant (infection stage), the general plant vigor (health), varietal susceptibility, conducive environment (viruses express better in colder weather than hot weather), a plant that has already been infected with other viruses (preesisting condition) are to name a few.
Attachment – the virus attaches itself to the outside of a new plant cell
Penetration – the protein pushes the nucleic acid strand into the plant cell
Replication – the viruses’ nucleic acid uses the plant cell DNA to make many new nucleic acid strands and protein sheathes
Assembly – the nucleic acid and protein assembly into millions of new virus copies
Release – the viruses leave the cell – at this stage the cell is normally dead and bursts releasing the viruses
Transmission – the viruses move using a vector to new cells to infect.
When you see the symptoms in your plants, the first thing you have to understand is virus infection is systemic. The best you can do to manage the virus is to limit the transmission (flatten the curve). Some viruses need a vector for transmission like insects and nematodes. Some viruses are mechanically transmitted from one infected plant to another. Washing field tools between plants/field whenever possible limits the transmission of virus. Soap, bleach, and disinfectants reduce transmission by protein denaturalization of the virus.
Controlling Fusarium Wilt of Lettuce Using Steam Heat – Trial Initiated
Earlier this week, we initiated a trial examining the use of band steam for controlling Fusarium wilt of lettuce. The premise behind this research is to use steam heat to raise soil temperatures to levels sufficient to kill soilborne pathogens. For Fusarium oxysporum f. sp. lactucae, the pathogen which causes Fusarium wilt of lettuce, the required temperature for control is generally taken to be > 140°F for 20 minutes. Soil solarization, where clear plastic is placed over the crop bed during the summer, exploits this concept. The technique raises soil surface temperatures to 150-155˚F, effectively killing the pathogen and reducing disease incidence by 45-98% (Matheron and Porchas, 2010).
In our trials, we are using steam heat to raise soil temperatures. Steam is delivered by a 35 BHP steam generator mounted on a custom designed elongated bed shaper (Fig. 1). Preliminary results were encouraging. The device was able to increase the temperature of the top 3” of soil to over 180°F at a travel speed of 0.5 mph as shown in this video of the machine in action (shown below). These temperatures exceed that of those known to control pathogens responsible for causing Fusarium wilt of lettuce (> 140°F for 20 minutes).
Stay tuned for final trial results and reports on the efficacy of using steam heat to control Fusarium wilt of lettuce.
If you are interested in evaluating the technique on your farm, please contact me. We are seeking additional sites with a known history of Fusarium wilt of lettuce disease incidence to test the efficacy and performance of the device.
References
Matheron, M. E., & Porchas, M. 2010. Evaluation of soil solarization and flooding as management tools for Fusarium wilt of lettuce. Plant Dis. 94:1323-1328.
Acknowledgements
This project is sponsored by USDA-NIFA, the Arizona Specialty Crop Block Grant Program and the Arizona Iceberg Lettuce Research Council. We greatly appreciate their support.
A special thank you is extended to Cory Mellon and Mellon Farms for allowing us to conduct this research on their farm.
Weeds are one of the most visible of all agricultural pests. They can’t move or hide and once established often stick up over the crop. Just one weed in a 10 acre field is annoying to look at. With insects and diseases, the damage is often more visible than the pest. That is not the case with weeds. A moderate weed infestation is approximately 10 weeds per square foot. If a herbicide produces 90% control, that leaves 1 weed per square foot or 43 weeds per acre. Without an untreated check, this can look like the herbicide failed! It is easy to leave an untreated spot in a field and it is well worth doing. Many applicators do so unintentionally because of skips, powerlines and other causes. They help determine crop injury and weed control. Here are some examples of what various levels of control looked like from one of our cole crop trials: