Historically, our Areawide Pheromone and Sticky Trap monitoring for insects was terminated around the first of April as the produce season ended. Beginning 2 years ago however, we continued our Areawide Trapping Network throughout the summer to collect trapping data from all 16 areawide trap locations year-round. So why is this additional trapping data useful? For several reasons. First, understanding the activity of some of our key pests when produce is not grown during the summer may give us an indication of what to expect as the fall produce season begins. This may be particularly helpful for predicting moth flights and whitefly flights in August-September coinciding with early transplanting and direct seeded crops. Another example is keeping track of corn earworm which can unexpectedly show up near the beginning of fall harvests. Secondly, trapping for pests during the summer has shown us that 2 of our more important produce pests are not caught in traps during the summer. We presume this is due to the absence of brassica crops and weeds for diamondback moth, and high daytime/nighttime temperatures lethal to aphids. The fact that trap catches resume in the fall supports our conclusion that these pests are absent in the summer, only to reenter the desert via winds and/or transplants in the fall. And finally, it gives me something to do in the summer. So, visit the Areawide Summer Trap Network if you’re curious what our key pests are up to. We also continue to monitor thrips activity during the summer. This involves plant sampling using a dislodgement sampling method (beat pan) to determine the relative abundance of thrips adults and larvae on alfalfa, cotton, melons, wheat, Sudan grass, and weeds. This is being conducted to supplement our yellow sticky trap data that only indicates thrips adult flight movement an area. With this plant sampling we should be able to determine the primary host plants thrips are colonizing in the cropping season during the long hot summer. We are also trying to determine whether these crops allow for the reproduction of thrips. So far, all the crops and weeds we have sampled have shown that thrips will reproduce and complete their life cycle on them. This is important, particularly for weeds, as we are trying to determine whether INSV can be survive the summer in the absence of lettuce. It is also important because it allows us to determine potential exposure of thrips to key insecticides like Radiant and Lannate for resistance management purposes. These crops essentially serve as untreated refugia that likely sustains insecticide susceptibility and is the reason these products are still effective against thrips. We will be conducting this work throughout the summer, as well as through next produce season. We will periodically provide updates of these thrips monitoring results throughout the summer. As my dad used to say, “to solve the problem, you must first understand the problem”.
Widely accepted definition of a living organism “A living organism has a cellular structure and is manifest by growth through metabolism, reproduction, and the power of adaptation to the environment through changes that originate internally”. Viruses are not cellular and do not metabolise, but they reproduce and adapt.
A virus is a set of one or more nucleic acid template molecules, normally incased in a protective coats of protein or lipoprotein and is able to organize its own replication but only within a suitable host cells. Record of plant viruses do not go as far as human viruses, but plant viruses have caused considerable loss in agriculture system.
One of the most common virus we see in agriculture system in todays world is Cucumber mosaic virus(CMV). CMV belongs to family Bromoviridae. The genome size of cucumber mosaic virus (see pic) is about 8000 to 9000 nucletotide bases (1 base=1 letter of AGTC). The genome size of Covid19 Coronivirus is about 30,000 bases and the genome size of human DNA is 6.4 billion bases.
CMV has a very wide host range and is transmitted by aphids in nonpersistent manner (stylet borne). This means that the aphids acquire the virus particle in their stylet within seconds of feeding in infected plants, hop on to next plant and start feeding on next plant. The virus is transmitted to the next plant immediately.
Next is incubation period. Viruses cause systemic infection. It can take anywhere from few days to few weeks from initial entry of the virus to symptom exhibition in your plants. The severity of symptoms varies depending on many factors. The age of plant (infection stage), the general plant vigor (health), varietal susceptibility, conducive environment (viruses express better in colder weather than hot weather), a plant that has already been infected with other viruses (preesisting condition) are to name a few.
Attachment – the virus attaches itself to the outside of a new plant cell
Penetration – the protein pushes the nucleic acid strand into the plant cell
Replication – the viruses’ nucleic acid uses the plant cell DNA to make many new nucleic acid strands and protein sheathes
Assembly – the nucleic acid and protein assembly into millions of new virus copies
Release – the viruses leave the cell – at this stage the cell is normally dead and bursts releasing the viruses
Transmission – the viruses move using a vector to new cells to infect.
When you see the symptoms in your plants, the first thing you have to understand is virus infection is systemic. The best you can do to manage the virus is to limit the transmission (flatten the curve). Some viruses need a vector for transmission like insects and nematodes. Some viruses are mechanically transmitted from one infected plant to another. Washing field tools between plants/field whenever possible limits the transmission of virus. Soap, bleach, and disinfectants reduce transmission by protein denaturalization of the virus.
Controlling Fusarium Wilt of Lettuce Using Steam Heat – Trial Initiated
Earlier this week, we initiated a trial examining the use of band steam for controlling Fusarium wilt of lettuce. The premise behind this research is to use steam heat to raise soil temperatures to levels sufficient to kill soilborne pathogens. For Fusarium oxysporum f. sp. lactucae, the pathogen which causes Fusarium wilt of lettuce, the required temperature for control is generally taken to be > 140°F for 20 minutes. Soil solarization, where clear plastic is placed over the crop bed during the summer, exploits this concept. The technique raises soil surface temperatures to 150-155˚F, effectively killing the pathogen and reducing disease incidence by 45-98% (Matheron and Porchas, 2010).
In our trials, we are using steam heat to raise soil temperatures. Steam is delivered by a 35 BHP steam generator mounted on a custom designed elongated bed shaper (Fig. 1). Preliminary results were encouraging. The device was able to increase the temperature of the top 3” of soil to over 180°F at a travel speed of 0.5 mph as shown in this video of the machine in action (shown below). These temperatures exceed that of those known to control pathogens responsible for causing Fusarium wilt of lettuce (> 140°F for 20 minutes).
Stay tuned for final trial results and reports on the efficacy of using steam heat to control Fusarium wilt of lettuce.
If you are interested in evaluating the technique on your farm, please contact me. We are seeking additional sites with a known history of Fusarium wilt of lettuce disease incidence to test the efficacy and performance of the device.
References
Matheron, M. E., & Porchas, M. 2010. Evaluation of soil solarization and flooding as management tools for Fusarium wilt of lettuce. Plant Dis. 94:1323-1328.
Acknowledgements
This project is sponsored by USDA-NIFA, the Arizona Specialty Crop Block Grant Program and the Arizona Iceberg Lettuce Research Council. We greatly appreciate their support.
A special thank you is extended to Cory Mellon and Mellon Farms for allowing us to conduct this research on their farm.
Weeds are one of the most visible of all agricultural pests. They can’t move or hide and once established often stick up over the crop. Just one weed in a 10 acre field is annoying to look at. With insects and diseases, the damage is often more visible than the pest. That is not the case with weeds. A moderate weed infestation is approximately 10 weeds per square foot. If a herbicide produces 90% control, that leaves 1 weed per square foot or 43 weeds per acre. Without an untreated check, this can look like the herbicide failed! It is easy to leave an untreated spot in a field and it is well worth doing. Many applicators do so unintentionally because of skips, powerlines and other causes. They help determine crop injury and weed control. Here are some examples of what various levels of control looked like from one of our cole crop trials: