Mar 23, 2022Insecticide Modes of Action and IRMTo contact John Palumbo go to: jpalumbo@ag.Arizona.edu
PATHOGEN: Pantoea ananatis, Pantoea agglomerans, Pantoea alli and Pantoea stewartii subsp. indologenes
HOSTS: Onion (Allium cepa L.), garlic (Allium sativum L.), shallots (Allium cepa var. aggregatum L.), leeks (Allium ampeloprasum L.), chives (Allium schoenoprasum L.).
Symptoms and signs
Center rot of onion has not been a major problem in the desert southwest but when the environment is favorable, the disease can cause up to 90% loss. Foliar symptoms (symptoms on leaves) may start with water-soaked lesions spanning the length of the leaf blade, which gradually become blighted resulting in desiccation and collapse of the tissue. Experiments have shown that the bacteria can move from leaves to the bulbs, thus protecting foliage is important to manage the disease.
The bacteria can overseason to infect onions in a number of different ways. Like many bacterial pathogens, P. ananatis can be seed-borne with infested seed serving as a survival mechanism as well as a means of dissemination. It has been demonstrated that P. ananatis can be both naturally seed-borne and seed-transmitted in onion. The significance of the bacterium's ability to colonize seed is uncertain, as most onion seed production sites are located in arid climates but extremely important to understand to manage the disease.
Although P. ananatis can be seedborne, the proposed primary mode of transmission is by two insect vectors. Two species of thrips, tobacco thrips (Frankliniella fusca (Hinds)) and onion thrips (Thrips tabaci), have the ability to transiently acquire and transmit P. ananatis and P. agglomerans . The bacterium can persist in a non-circulative manner in the gut of thrips for 128 h, allowing the vector to infect plants over an extended period of time.
P. ananatis can survive epiphytically and endophytically on a wide range of hosts. These alternative hosts can serve as a source of inoculum in fields where susceptible crops are grown. In Georgia alone, 25 weed species, including carpetweed (Mollugo verticillata), common ragweed (Ambrosia artemisiifolia), crabgrass (Digitaria sanguinalis), common cocklebur (Xanthium pensylvanicum), curly dock (Rumex crispus), Florida pusley (Richardia scabra), sicklepod (Cassia obtusifolia), stinkweed (Thlaspi arvense), Texas panicum (Panicum texanum), vaseygrass (Paspalum urvillei), wild radish (Brassica spp.), yellow nutsedge (Cyperus esculentus) and other multiple crop plants were found to harbor P. ananatis populations asymptomatically.
Pic Credit: Colton Tew
Center rot management in onion fields relies heavily on copper applications mixed with an ethylenebisdithiocarbamate fungicide (EBDC), such as mancozeb, which growers may apply weekly as a protectant. In addition, researchers found P. ananatis strains to be copper-tolerant indicating overuse and potential risk of insensitivity to this chemistry. Repeated applications of copper sprays during susceptible growth stages can be effective only to a limited extent and does not offer a robust solution to the problem. Perhaps the inefficacy of these sprays could be due to thrips preference to colonize certain parts of the onion plant, e.g. the basal meristems (neck region).
The implementation of successful weed management strategies are important in reducing P. ananatis inoculum in the field. By reducing weeds, growers can potentially reduce initial inoculum.
Last fall, we established two trials investigating the used of band-steam to control Fusarium wilt of lettuce. We utilized the prototype band-steam applicator (Fig 1) described in previous UA Veg IPM articles (Vol. 11 (15) to inject steam into the soil prior to planting. The concept behind band-steam is to disinfest narrow bands of soil using high temperature steam. In the trials, the steam applicator was configured to treat a 4” wide by 4” deep band of soil centered on the seedline.
Experiment results were mixed. At the field site where Fusarium inoculum loads were high, band-steam provided no benefit with virtually all lettuce plants succumbing to the disease (Fig 2a). However, at the trial site where Fusarium inoculum levels were moderate, disease incidence was reduced by more than 40%, and plants appeared to be healthier and more vigorous (Fig. 2b). We’ll be harvesting these plots soon so stay tuned to learn whether these differences translate into significant yield increases.
If you are interested in trying band-steam on your farm, please let me know. We are in the process of constructing a second-generation band-steam applicator that has a higher capacity steam generator and simpler design than our first prototype and are seeking collaborators.
This work is partially funded by the Arizona Specialty Crop Block Grant Program
A special thank you is extended to Larry Ott and Gila Valley Farms for allowing us to conduct this research on their farm.
Fig. 1. Band-steam applicator principally comprising a 35 BHP steam generator mounted on a bed-shaper applicator sled.
Fig. 2. Lettuce seedlings at field sites with (a) very high and (b) moderate levels of Fusarium wilt of lettuce inoculum. Band-steam (left) and untreated (right) plots are shown.