Sep 2, 2026
A reminder of the importance of environment on plant symptom development
Galls observed on the stems of begonias submitted to the clinic
Recently, we received a submission of begonias to the Yuma Plant Health Clinic that immediately presented a diagnostic challenge. At first glance, the symptoms seemed to point toward a familiar set of suspects. The plants had developed severe swelling of the stems, symptoms characteristic of physiological oedema or bacterial Agrobacterium-associated crown gall. But, as is often the case in plant diagnostics, appearances can be deceiving.
The begonias had been grown indoors in high-humidity terrariums and planter boxes. The symptoms were not associated with a particular begonia variety, nor were they consistently expressed across all plantings or cuttings. When the plants were removed from their planting substrate, however, another clue emerged: additional small galls were scattered throughout the root systems of those plants also showing the stem swellings. That observation changed the diagnostic picture considerably.
Galls observed on the roots of begonias submitted to the clinic
Dissection of both the swollen stem tissue and the roots revealed an abundance of eggs, juveniles, mature female nematodes, and giant cells, a classic signature of root-knot nematode (RKN) infection. Root-knot nematodes belong to the genus Meloidogyne, a group of microscopic roundworms that are among the most important plant-parasitic nematodes worldwide. Their typical symptom is familiar to many resulting in the formation of characteristic galls on roots.
These galls are not simply a physical wound created by the nematodes. Rather, RKNs actively manipulate their host. During infection, the nematodes secrete salivary compounds that alter plant growth and development, effectively hijacking normal cellular processes and redirecting them toward the formation of specialized feeding structures known as giant cells.
These giant cells become a nutrient-rich feeding site for the developing and mature nematodes. In doing so, the nematode establishes a sustained drain on the plant's resources. As infection progresses, the resulting disruption to the root system can interfere with nutrient acquisition and water uptake. Aboveground, this typically translates into stunted growth, declining foliage, wilting, and, in severe infestations, plant death. With more than 3,000 plant species reported as hosts to different root-knot nematode species worldwide, including many field and vegetable crops, the annual impact of these pathogens is enormous. Much research has been done on RKN in various crops, and reports of stem swellings occurring naturally is almost non-existent.
Yet the begonia case raises an especially interesting question: Why did a pathogen best known for producing root galls appear to be responsible for such dramatic swelling in the stems? This is where the environment becomes an essential part of the diagnosis.
The symptom is not the pathogen. It is a reaction of the plant to the presence of a pathogen. One of the most important principles in plant pathological diagnostics is that the same pathogen does not necessarily produce the same symptom under every set of environmental conditions. Likewise, similar symptoms can arise from entirely different causes.
Temperature, humidity, water availability, substrate conditions, light, nutrition, plant age, and even the way a plant is cultivated can all influence how a disease develops and how its symptoms are expressed. In other words, the symptom we see is the product of an interaction between the host, the pathogen, and the environment. Change any one of those components, and the resulting disease can change dramatically. This recent begonia case is a particularly useful reminder of this principle.
Root-knot nematodes are well known for their belowground symptoms, but reports of rare and isolated cases of infections on aerial plant tissues, including stems, leaves, and flowers, are reported in only one publicly available extension bulletin from 1985. These symptoms remain unusual and have rarely been observed unless aerial tissues are artificially inoculated, and they have only been documented in a limited number of hosts.
So, when confronted with the unusual stem symptoms in a begonia, it would be easy to focus exclusively on the appearance of the tissue and pursue diagnoses such as oedema or crown gall. These plants were being cultivated in enclosed, high-humidity conditions, where moisture levels around the plant were substantially different from those experienced by a plant growing in a more open environment. Such conditions can alter transpiration rates, water movement, tissue hydration, pathogen development, and the plant's own physiological responses. They also give rise to the wet, humid conditions necessary for oedema to develop or crown gall bacteria to thrive.
The small root galls were an important clue. Microscopic examination then provided the confirmation: eggs, juveniles, mature females, and the characteristic giant cells associated with root-knot nematode feeding. These signs of RKN were found in both the root and stem swellings. Understanding the environment in which the hosts were being grown, with its high moisture conditions, then provided supporting evidence that the nematodes had favorable environmental conditions to migrate into much higher foliar tissues than is typically seen in RKN infestations and produce an unusual symptom. Swollen galls on the stem told us that something was wrong, and if we relied on the most common culprits associated with this symptom alone, namely oedema or crown gall, we would have gotten the diagnosis wrong.
The begonia case is a fascinating example of how flexible disease expression can be. A pathogen with a reputation for being almost exclusively soilborne and producing galling symptoms on root tissues, under particular host and environmental circumstances, can be associated with symptoms in tissues where we do not ordinarily expect to find them.
Ultimately, environment is not simply a backdrop against which plant disease occurs. It is one of the determinants of how disease develops, how severe it becomes, and critically for anyone trying to ID the pathogenic cause, what it looks like.
If you have any concerns regarding the health of your plants/crops please consider submitting samples to the Yuma Plant Health Clinic for diagnostic service or booking a field visit with me:
Christopher Detranaltes, Ph.D.
Cooperative Extension – Yuma County
Email: cdetranaltes@arizona.edu
Cell: 602-689-7328
6425 W 8th St Yuma, Arizona 85364 – Room 109
