May 5, 2021Summer Sanitation Is Important as Ever
To contact John Palumbo go to: jpalumbo@ag.Arizona.edu
In the past couple of weeks, the reports of INSV in fields has increased dramatically. INSV has been found in fields in Yuma/Gila Valley, Wellton, Tacna, Roll, and Imperial Valley.
PCAs have reported thrips pressure as low this year and most fields have infection less than 1% but some fields have been reported to have higher incidence. The virus has been detected in direct seeded field as well as transplants imported from Salinas, CA.
Impatiens necrotic spot virus, also known as INSV is a tospovirus closely related to Tomato spotted wilt virus. Infected plants usually have leaves with brown to dark brown necrotic areas. Sometimes the symptoms may be confused with “chemical burn”. As necrosis progresses the leaf browns or die out. Plants infected in early stage may become stunted and die, or become unmarketable.
What makes this virus of high economic importance?
The plants become unmarketable which is the ultimate economic loss. But there are factors that facilitate the virus outbreak.
The first one is efficient transmission by its vector (s). The virus is transmitted by western flower thrips, Frankliniella occidentalis.
If you remember our virus transmission series in past newsletters, thrips transmit viruses in persistent propagative manner. Insects have to feed on virus infected plants for hours/days to acquire the virus and the virus has to incubate for hours/days in the insect. After insect can transmit the virus throughout its lifespan. The virus can multiply in the vector system and often times the virus particles are also passed on to the insect offspring. Adult thrips can transmit these viruses only if acquired in the larval stage of development. Larval thrips will feed on a virus-infected plant, pupate, and emerge as a winged adult capable of transmitting the virus. The thrips then will carry the virus for life.
The next contributing factor is host range. INSV infects large number of ornamental and vegetable plants. We are talking 600 species of plants that are susceptible to INSV and thrips love flowers.
While it may not be practical to remove all your ornamentals in fear of INSV, it is definitely practical to monitor thrips population in your field. As the legend says “When in doubt, scout”.
And if you need diagnosis, drop the samples in the clinic! But then if you have immunostrips, you don’t have to make the drive to the Ag Center!
DIY testing: Impatiens necrotic spot virus (INSV)
As visual diagnosis of the virus is confusing and could even be misleading at times, it is very important to confirm a symptomatology via clinical diagnosis.
The good news is there are tools available for quick and easy diagnosis of INSV. You can order the immunostrips from Agdia (https://orders.agdia.com/agdia-immunostrip-for-insv-isk-20501)
The immunostrips cost anywhere from $5-20 depending on how much you buy. They perform better when they stay refrigerated until just before use.
Immunostrips are quick and easy tool to use. The kit comes with a buffer bag and immunostrip.
One band means that the positive control worked which means the system worked. Sometimes you see no bands at all. This means the system did not work and you have to repeat the test.
If you are seeing symptoms in your field please let Bindu Poudel-Ward know via email (firstname.lastname@example.org or text (928-920-1110). Please keep a note of weed species you are consistently seeing in your fields and keep the thrips population under check.
Mark C. Siemens
Vol. 12, Issue 9, Published 5/5/2021
Automated thinning machines have been commercially available since 2012. These machines identify crop plants and intermittently deliver an herbicidal spray or dose of liquid fertilizer to thin the stand to the desired plant spacing. Some growers have converted older machines to spot apply pesticides to crop plants rather than thin lettuce. Spot spraying just the crop plant makes sense – it reduces applied chemical amount by about 1/3rd as compared to band spraying and by roughly 90% as compared to broadcast. I have heard reports of improved efficacy with this technique, perhaps due to better coverage, however this potential benefit has not been validated in formal trials.
A drawback with automated thinning machines is their high cost. Retail prices for machines are approximately $25,000 per seed line, or about $200,000 for a 4-row, 2-line machine. Another option might be to use automated systems designed for spot spraying weeds. These devices have been commercially available since the mid 90’s and function similarly to automated thinning machines in that they use optical sensors to detect plants and solenoid activated spray assemblies to intermittingly spray unwanted plants (Fig. 1). The cost of these devices is quite reasonable – about $3,000 per unit, or about $24,000 for a 4-row, 2-line machine.
Automated spot sprayers are typically used in agriculture to control weeds in fallow fields (Fig. 2), but could easily be adapted to apply pesticides or even fertilizer to vegetable crops. Spot applying foliar fertilizers to vegetable crops is an interesting concept and is being investigated in California with lettuce.
Another potential use of spot sprayers is to control herbicide resistant weeds. The device can be positioned between crop rows to spot spray a non-selective herbicide to target weeds. Placing the sprayer in a hooded enclosure prevents unwanted drift onto crop plants. We are conducting trials using this technique in cotton this season (Fig. 3). We are also looking for collaborators interested in trying the device as a pesticide and/or fertilizer spot applicator in vegetable crops for this upcoming season. If you are interested collaborating or would like to see a demo of the device, please feel free to reach out to me.
Growers and PCAs can monitor data from the Yuma Leaf Wetness Network through the AZMET website located at the following URL: http://126.96.36.199:460
The website updates information on leaf wetness and near-surface air temperature every 15 minutes. Wetness data are provided in graphical format (see figure below). Output from the leaf wetness sensors increase from the grey (dry) zone of the graph to the blue (wet) zone when wetness (dew or rain) is detected by the sensors.