Jun 10, 2026
Effects of the Maximum Dose of Common Insecticides and Incipio on Diamondback Moth Populations Collected from Arizona and California Brassica Crops (Fall 2025 – Winter/Spring 2026)
Diamondback moth (DBM), Plutella xylostella, is one of the most important insect pests of Brassica crops, including cabbage, broccoli, cauliflower, and some leafy greens. Heavy infestations can cause significant feeding damage, reduce crop quality, and increase production costs. Because DBM has a long history of developing resistance to insecticides, regular monitoring of insecticide susceptibility is essential to ensure effective management in the field. In the western United States, resistance to several key insecticide classes has been documented in field populations. For example, populations in California have shown high levels of resistance to several insecticide classes, including diamides, spinosyns, avermectins, pyrethroids, and Bacillus thuringiensis (Bt) products. More recently, outbreaks in Arizona have been associated with confirmed resistance to diamide insecticides and reports of reduced field efficacy. This highlights the importance of regular insecticide susceptibility monitoring and the implementation of resistance management strategies to maintain effective control of the pest. While field resistance and reduced efficacy reflect outcomes under production conditions, laboratory susceptibility monitoring provides a standardized approach to detect shifts in population response before or alongside observable field control issues.
To evaluate current insecticide susceptibility levels in DBM populations across Arizona's major Brassica-growing regions and some California populations, DBM larvae were collected from multiple locations in Arizona and California. DBM populations were reared in the laboratory, and their progenies were tested under controlled laboratory conditions.
In the laboratory, larvae were exposed to insecticides at the highest labeled field rate using a leafdip bioassay. The insecticides evaluated included Exirel®, Coragen®, Harvanta®, Radiant®, Baythroid® XL, Proclaim®, Torac®, DiPel®, and XenTari®, representing commonly used conventional and organic management options, as well as IncipioTM, which was recently registered. These laboratory bioassays measure relative susceptibility under controlled conditions using field-collected populations reared in the laboratory. Results represent mortality at the maximum labeled rate under leaf-dip assay conditions and may not directly reflect field performance, where environmental conditions, application timing, coverage, larval stage, and population dynamics influence control. These data are intended to monitor susceptibility trends and help inform resistance management decisions. Accordingly, extrapolation to field performance should be made cautiously, recognizing both the constraints of laboratory bioassays and the mosaic of susceptibility that may exist within and among field populations.
Several Insecticides Showed High Laboratory Mortality Across Field-Collected Populations
Several of the insecticides evaluated caused high mortality of DBM larvae in laboratory assays across field populations. Proclaim, Incipio, Radiant, DiPel, and XenTari caused high mortality, indicating laboratory susceptibility across the tested populations. These products remain promising options for managing DBM in Brassica crops based on current laboratory susceptibility data. In contrast, Coragen, Exirel, and Baythroid XL resulted in lower mortality in laboratory assays than the other insecticides (Figure 1).

Figure 1. Mean mortality (%) of DBM larvae exposed to the maximum label rate of
insecticides, combined across 10 field populations from California and Arizona.
DBM Mortality by Collection Location, with Some Population-Level Variability
The levels of larval mortality caused by each evaluated insecticide were generally consistent across populations. For DBM populations collected during fall 2025, Proclaim, Incipio, Radiant, DiPel, and XenTari resulted in high larval mortality under laboratory assay conditions for most DBM populations tested (Figure 2). However, slight reductions in larval mortality were observed in specific populations. For example, DiPel resulted in approximately 70% larval mortality in the Coachella Valley, CA population # 4, and Radiant caused approximately 55% larval mortality in the Coachella Valley, CA population #2. Despite this variability, these insecticides generally resulted in high mortality of DBM larvae across geographic locations under laboratory assay conditions (see Figure 1). In contrast, Coragen, Exirel, and Baythroid XL consistently resulted in low DBM larvae mortality in the field-collected populations from Salinas Valley, CA; Coachella Valley, CA; and Gila Valley, AZ (Figure 2).
We observed similar mortality trends among populations collected during winter/spring 2026 for the evaluated insecticides except for DBM mortality caused by Dipel, which declined in many populations (Figures 3 and 4). Harvanta was evaluated for two populations (Imperial Valley, CA and Somerton, AZ), which exhibited similar reduced larval mortality to that of the other diamides. Torac was also evaluated for the Imperial Valley, CA, population, resulting in a high level of DBM mortality (Figure 4). We also tested a population collected from the Yuma Ag Center, which resulted in susceptibility levels very similar to those of the susceptible laboratory colony (Figure 3). Previous DBM monitoring studies performed by Dr. Palumbo over several years also showed that while populations collected from commercial fields showed very low susceptibility to diamides, populations collected at the Yuma Ag Center remained highly susceptible. This indicates that transplant sources and/or field-specific DBM management practices are also affecting DBM susceptibility and control across Arizona’s brassica-growing regions.
The reduced and variable levels of larval mortality observed in laboratory assays across multiple field populations for some tested insecticides highlight the importance of routine susceptibility monitoring to better understand resistance dynamics and to support informed field-level decisions regarding product selection and rotation.

Figure 2. Mortality (%) of DBM larvae from field populations collected from Arizona
and California as affected by maximum label rates of 8 insecticides, Fall 2025.

Figure 3. Mortality (%) of DBM larvae from a susceptible lab colony and field
populations collected from Arizona as affected by maximum label rates of 8
insecticides, Winter/Spring 2026.

Figure 4. Mortality (%) of DBM larvae from two field populations collected from
Arizona and California as affected by maximum label rates of ten insecticides, Spring 2026.
Key Laboratory Findings
- Several products resulted in consistently high larval mortality under laboratory assay conditions across field-collected populations.
- Some products resulted in comparatively lower larval mortality in laboratory assays across multiple field-collected populations.
- Mortality levels varied among certain DBM populations, suggesting differences in susceptibility.
Implications for Resistance Monitoring and Field Decision-Making
- Continued susceptibility monitoring is important for detecting shifts in population response before widespread field control issues emerge.
- Product rotation among different modes of action remains an important tactic to slow resistance development.
Laboratory monitoring informs our understanding of resistance trends, but field scouting remains essential to evaluate how populations respond under production conditions. As always, when in doubt, scout!
Additional Reading Materials
- Calvin W., M. N. Keith, and B. McGrew. 2025. Guidelines for Effective Management of Diamondback Moth in Brassica Crops. University of Arizona Extension Publication. az2143. https://extension.arizona.edu/publication/guidelines-effective-management-diamondbackmoth-brassica-crops
- Calvin, W. and J. C. Palumbo. 2024. Chlorantraniliprole Resistance Associated with Diamondback Moth (Lepidoptera: Plutellidae) Outbreaks in Arizona Brassica Crops. Journal of Economic Entomology. toae212, https://doi.org/10.1093/jee/toae212.