Mar 4, 2026
Effects of the Maximum Dose of Common Insecticides and Incipio on Diamondback Moth Populations Collected from Arizona and California Brassica Crops
Diamondback moth (DBM), Plutella xylostella, is one of the most important insect pests of Brassica crops, including cabbage, broccoli, cauliflower, and some leafy greens. Heavy infestations can cause significant feeding damage, reduce crop quality, and increase production costs. Because DBM has a long history of developing resistance to insecticides, regular monitoring of insecticide susceptibility is essential to ensure effective management in the field. In the western United States, resistance to several key insecticide classes has been documented in field populations. For example, populations in California have shown high levels of resistance to several insecticide classes, including diamides, spinosyns, avermectins, pyrethroids, and Bacillus thuringiensis (Bt) products. More recently, outbreaks in Arizona have been associated with confirmed resistance to diamide insecticides and reports of reduced field efficacy. This highlights the importance of regular insecticide susceptibility monitoring and the implementation of resistance management strategies to maintain effective control of the pest. While field resistance and reduced efficacy reflect outcomes under production conditions, laboratory susceptibility monitoring provides a standardized approach to detect shifts in population response before or alongside observable field control issues.
To evaluate current insecticide susceptibility levels in DBM populations across Arizona's major Brassica-growing regions and some California populations, DBM larvae were collected from multiple locations in Arizona and California. DBM populations were reared in the laboratory and their progenies were tested under controlled laboratory conditions. Only one Arizona population was included in this initial assessment. Additional populations from Arizona have since been collected and are currently being tested to provide a more comprehensive understanding of insecticide susceptibility across the region.
In the laboratory, larvae were exposed to insecticides at the highest labeled field rate using a leaf-dip bioassay. The insecticides evaluated included Exirel®, Coragen®, Radiant®, Baythroid® XL, Proclaim®, IncipioTM, DiPel®, and XenTari®, representing commonly used conventional and organic management options, as well as IncipioTM, which was recently registered. These laboratory bioassays measure relative susceptibility under controlled conditions using field-collected populations reared in the laboratory. Results represent mortality at the maximum labeled rate under leaf-dip assay conditions and may not directly reflect field performance, where environmental conditions, application timing, coverage, larval stage, and population dynamics influence control. These data are intended to monitor susceptibility trends and help inform resistance management decisions. Accordingly, extrapolation to field performance should be made cautiously, recognizing both the constraints of laboratory bioassays and the mosaic of susceptibility that may exist within and among field populations.
Several Insecticides Showed High Laboratory Mortality Across Field-Collected Populations
Several of the insecticides evaluated caused high mortality of DBM larvae in laboratory assays across field populations. Proclaim, Incipio, Radiant, DiPel, and XenTari caused high mortality, indicating laboratory susceptibility across the tested populations. These products remain promising options for managing DBM in Brassica crops based on current laboratory susceptibility data.
In contrast, Coragen, Exirel, and Baythroid XL resulted in lower mortality in laboratory assays than the other insecticides (Figure 1).

Figure 1. Mean mortality (%) of DBM larvae exposed to the maximum label rate of
insecticides, combined across all six field populations from California and
Arizona.
Consistent Laboratory Mortality Across Regions, with Some Population-Level Variability
The levels of larval mortality caused by each evaluated insecticide were generally consistent across populations collected from major Brassica-growing regions, including Salinas Valley, CA; multiple sites in Coachella Valley, CA; and Gila Valley, AZ (Figure 2). Proclaim, Incipio, Radiant, DiPel, and XenTari resulted in high larval mortality under laboratory assay conditions for most DBM populations tested. However, slight reductions in larval mortality were observed in specific populations. For example, DiPel resulted in approximately 70% larval mortality in the Coachella Valley, CA population # 4, and Radiant caused approximately 55% larval mortality in the Coachella Valley, CA population #2. Despite this variability, these insecticides generally resulted in high mortality of DBM larvae across geographic locations under laboratory assay conditions (see Figure 1). In contrast, Coragen, Exirel, and Baythroid XL resulted in high larval mortality for the laboratory strain but consistently resulted in low DBM larvae mortality in the field-collected populations from Salinas Valley, CA; Coachella Valley, CA; and Gila Valley, AZ (Figure 2).
The reduced and variable levels of larval mortality observed in laboratory assays across multiple field populations for some tested insecticides highlight the importance of routine susceptibility monitoring to better understand resistance dynamics and to support informed field-level decisions regarding product selection and rotation.

Figure 2. Mortality (%) of DBM larvae from laboratory and field populations
exposed to maximum label rates of insecticides.
Key Laboratory Findings
- Several products resulted in consistently high larval mortality under laboratory assay conditions across field-collected populations.
- Some products resulted in comparatively lower larval mortality in laboratory assays across multiple field-collected populations.
- Mortality levels varied among certain populations, suggesting heterogeneity in susceptibility.
Implications for Resistance Monitoring and Field Decision-Making
- Continued susceptibility monitoring is important for detecting shifts in population response before widespread field control issues emerge.
- Product rotation among different modes of action remains an important tactic to slow resistance development.
Laboratory monitoring informs our understanding of resistance trends, but field scouting remains essential to evaluate how populations respond under production conditions. As always, when in doubt, scout!
Additional Reading Materials
1. Calvin W., M. N. Keith, and B. McGrew. 2025. Guidelines for Effective Management of Diamondback Moth in Brassica Crops. University of Arizona Extension Publication. az2143. https://extension.arizona.edu/publication/guidelines-effective-management-diamondback-moth-brassica-crops
2. Calvin, W. and J. C. Palumbo. 2024. Chlorantraniliprole Resistance Associated with Diamondback Moth (Lepidoptera: Plutellidae) Outbreaks in Arizona Brassica Crops. Journal of Economic Entomology. toae212, https://doi.org/10.1093/jee/toae212.